IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-7-20

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To-do

  • Reinoculate the Hetmann Six cultures
  • Run and image gels of the colony PCR we did last night
  • Send CY013 and CY014 to Genewiz again
  • Transform TP (the original Topo + KaiABC plasmid) to grow up more


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L; LC1 raw; LC2 raw; LC2 pure (1); LC2 pure (10); PL1; PL2; LC1 pure (10); LC1 pure (1); (-); L

Retransformation of our first Topo + KaiABC plasmid

I retransformed our first miniprepped Topo + KaiABC plasmid since we didn't have enough plasmid to use for sequencing (Geneqiz didn't receive our order modification in time).