IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-7-20

To-do

• Reinoculate the Hetmann Six cultures
• Run and image gels of the colony PCR we did last night
• Send CY013 and CY014 to Genewiz again
• Transform TP (the original Topo + KaiABC plasmid) to grow up more

L; LC1 raw; LC2 raw; LC1 pure (1); LC2 pur (1); LC1 pure (10); L21 pure (10); PL1; PL2; (-); L

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L; LC1 raw; LC2 raw; LC2 pure (1); LC2 pure (10); PL1; PL2; LC1 pure (10); LC1 pure (1); (-); L

Nick's tips for gels

• When microwaving, be certain that the broth does not boil for too long, because this will evaporate the water and the % of agarose will be higher for the gel
  • Take the flask out when this happens and gently swirl
• Make sure that the comb and the trays are clean
• Let the flask cool to a temperature comfortable to hold in your palm
• Pour gently to avoid bubbles
• When there are bubbles in the gel, use the larger end of a pipet tip to scoop the bubble out
• Cover the gel with foil to avoid bugs

Retransformation of our first Topo + KaiABC plasmid

I retransformed our first miniprepped Topo + KaiABC plasmid since we didn't have enough plasmid to use for sequencing (Geneqiz didn't receive our order modification in time).