IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-7-21
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To-do
- Inoculate liquid cultures with retransformed TP strain from yesterday-- done
- Extract and purify LC1PUR1 and LC2PUR2 (3kb and 400b bands) from PCR Extraction #5 of 2006-7-19.
- Ligate gel-purified PCR product with Topo, transform with Top10 competent cells, spread plates
- Wiki cleanup
- Update incubator status
- Work on Monday presentation outline
- Send out signed synthesis contract with GeneArt
Strike three
Once again we left a bucket of ice out on the benchtop overnight. This one contained Invitrogen's Zero-Blunt Topo cloning kit. We've labeled the box as possibly bad.
Synthesis contract
- Sign contract (3x)
- Billing - mailto address needs to be changed
- Add comment to ship as soon as ready
Inoculation
- Each of the plates that were transformed from last night had lots of growth.
- One inoculation was made for each of the plates.
- One colony was taken from each of the plates to prevent mixes of colonies being grown up.
- The inoculations consisted of:
- 5 mL LB liquid culture
- 2 um Kan
- one colony
Gel extraction
We extracted 4 different bands from the gel that we ran yesterday:
- LC1 3kb fragment
- 85mg
- LC1 400bp fragment
- 197.5mg
- LC2 3kb fragment
- 132.9mg
- LC2 400bp fragment
- 213.3mg
- LC1 3kb fragment
