IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-7-22: Difference between revisions
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JeffreyLau (talk | contribs) (→To-do) |
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# Transform the ligated Topo + KaiABC and Topo + 400b with Top10 competent cells | # Transform the ligated Topo + KaiABC and Topo + 400b with Top10 competent cells | ||
# Plate | # Plate | ||
==Refrigerator open== | |||
The fridge door was open when I came in. There was a big puddle of something leaking out the bottom, which I cleaned up with towels. | |||
Revision as of 15:14, 22 July 2006
To-do
- Make frozen stocks of the retransformed Topo + KaiABC (original strain)
- Make a 20% glycerol mix (40% volume of 50% glycerol, 60% volume of culture), a little under 2 mL total volume
- Miniprep the rest
- Ligate the purified PCR product from PCR extraction #5. There are 4 reactions: LC1PUR1 3kb, LC1PUR1 400b, LC2PUR1 3kb, LC2PUR1 400b
- Transform the ligated Topo + KaiABC and Topo + 400b with Top10 competent cells
- Plate
Refrigerator open
The fridge door was open when I came in. There was a big puddle of something leaking out the bottom, which I cleaned up with towels.
