IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-14

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{{IGEM:/Harvard/2006/Cyanobacteria}}
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<li>[[IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-13 | Previous Entry]]</li>
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<li>[[IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-14 | Current Entry]]</li>
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==E-gel images of colony PCR of J04500 + KaiA/B/C ==
==E-gel images of colony PCR of J04500 + KaiA/B/C ==
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It appears that our vector self-ligated.
It appears that our vector self-ligated.
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==To-do==
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*#Midiprep pSB4A, J04500
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*Insert:
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*#Digest miniprepped KaiA/B/C in GeneArt plasmid with X-P
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*bkb:
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*#Digest J04500\S-P, \X-P (for Stage I constructs and BB high-copy)
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*KaiABC ligation:
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*#KaiABC\X-P + J04500\S-P (stage I)
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*#KaiABC\X-P + pSB4K3 (well-formed BB)
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*Sequencing:
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*#Sequence miniprepped KaiA/B/C + GA vectors, natural KaiA
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*Western blots:
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*#Ligate GFP dev and pSB4A3
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*#Inoculate GFP dev + pSB1A2

Revision as of 12:30, 14 August 2006




E-gel images of colony PCR of J04500 + KaiA/B/C

Click for legend
Click for legend
Click for legend
Click for legend


It appears that our vector self-ligated.

To-do

    1. Midiprep pSB4A, J04500
  • Insert:
    1. Digest miniprepped KaiA/B/C in GeneArt plasmid with X-P
  • bkb:
    1. Digest J04500\S-P, \X-P (for Stage I constructs and BB high-copy)
  • KaiABC ligation:
    1. KaiABC\X-P + J04500\S-P (stage I)
    2. KaiABC\X-P + pSB4K3 (well-formed BB)
  • Sequencing:
    1. Sequence miniprepped KaiA/B/C + GA vectors, natural KaiA
  • Western blots:
    1. Ligate GFP dev and pSB4A3
    2. Inoculate GFP dev + pSB1A2
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