IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-16: Difference between revisions

Revision as of 09:30, 16 August 2006

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Western blots
- ~~Inoculate several cultures of GFP dev. for Western blotting this afternoon~~
- Take regular measurements of culture OD
- Western blot
Construct creation
- Midiprep the KaiA/B/C in GA plasmid that we grew up from frozen stocks yesterday
  - Digest a large amount of KaiA/B/C\X-P
  - Gel-purify
- RBS + KaiC construct
  - Miniprep the B0034 cultures
  - Digest B0034\S-P
  - Ligate KaiC\X-P + B0034\S-P
  - Transform KaiC\X-P + B0034\S-P

10 inoculations of the R0010 + E0241 were made:

@@ Line 19: / Line 19: @@
 ** Midiprep the KaiA/B/C in GA plasmid that we grew up from frozen stocks yesterday
 *** Digest a large amount of KaiA/B/C\X-P
+*** Gel-purify
 ** RBS + KaiC construct
 *** Miniprep the B0034 cultures