IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-16: Difference between revisions
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==To-do== | == To-do == | ||
* Western blots | * Western blots | ||
** <s>Inoculate several cultures of GFP dev. for Western blotting this afternoon</s> | ** <s>Inoculate several cultures of GFP dev. for Western blotting this afternoon</s> | ||
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*** Transform KaiC\X-P + B0034\S-P | *** Transform KaiC\X-P + B0034\S-P | ||
==Inoculation R0010 + E0241== | == Inoculation R0010 + E0241 == | ||
10 inoculations of the R0010 + E0241 were made: | 10 inoculations of the R0010 + E0241 were made: | ||
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:* 10 mL LB amp & 4.1 mL R0010 + E0241 (IPTG - not yet added) | :* 10 mL LB amp & 4.1 mL R0010 + E0241 (IPTG - not yet added) | ||
==Midiprep of KaiABC== | == Midiprep of KaiABC == |
Revision as of 09:31, 16 August 2006
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To-do
- Western blots
Inoculate several cultures of GFP dev. for Western blotting this afternoon- Take regular measurements of culture OD
- Western blot
- Construct creation
- Midiprep the KaiA/B/C in GA plasmid that we grew up from frozen stocks yesterday
- Digest a large amount of KaiA/B/C\X-P
- Gel-purify
- RBS + KaiC construct
- Miniprep the B0034 cultures
- Digest B0034\S-P
- Ligate KaiC\X-P + B0034\S-P
- Transform KaiC\X-P + B0034\S-P
- Midiprep the KaiA/B/C in GA plasmid that we grew up from frozen stocks yesterday
Inoculation R0010 + E0241
10 inoculations of the R0010 + E0241 were made:
- 10 mL LB amp & 1 ul R0010 + E0241
- 10 mL LB amp & 1 ul R0010 + E0241 (IPTG - not yet added)
- 10 mL LB amp & 8 ul R0010 + E0241
- 10 mL LB amp & 8 ul R0010 + E0241 (IPTG - not yet added)
- 10 mL LB amp & 64 ul R0010 + E0241
- 10 mL LB amp & 64 ul R0010 + E0241 (IPTG - not yet added)
- 10 mL LB amp & 256 ul R0010 + E0241
- 10 mL LB amp & 256 ul R0010 + E0241 (IPTG - not yet added)
- 10 mL LB amp & 4.1 mL R0010 + E0241
- 10 mL LB amp & 4.1 mL R0010 + E0241 (IPTG - not yet added)