IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-17: Difference between revisions
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[[Image: 2006-8-17_Digest_Gel_Image.jpg|thumb|left|80V for 90 min<br>''Click for Legend'']] | [[Image: 2006-8-17_Digest_Gel_Image.jpg|thumb|left|80V for 90 min<br>''Click for Legend'']] | ||
[[Image:2006-08-17 21hr 53min.jpg|thumb|left|80V for 105 min<br>"Click for Legend"]] | [[Image:2006-08-17 21hr 53min.jpg|thumb|left|80V for 105 min<br>"Click for Legend"]] | ||
==Ligation and Transformation attempt #3 for stage 1 of constructs== | |||
This ligation and transformation attempt was done for 9 things: | |||
*B0034\sp + KaiA \xp, KaiB \xp, KaiC \xp | |||
*J4500\xp from the 97.3 ng/uL midiprep + KaiA \xp, KaiB \xp, KaiC \xp | |||
*J4500\xp from the 97.3 ng/uL midiprep + KaiA \xp, KaiB \xp, KaiC \xp | |||
Did a 1:3 volume ratio because the gel cutout did not give good nanodrop numbers; the usual, 25uL competent cells per sample, plated 5 on KAN (the J4500 \xp), 5 on Carb (the B0034 and the controls), and 1 of the J4500\xp on KAN (the KaiC \xp). | |||
Revision as of 17:48, 19 August 2006
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Click for Legend
"Click for Legend"
Ligation and Transformation attempt #3 for stage 1 of constructs
This ligation and transformation attempt was done for 9 things:
- B0034\sp + KaiA \xp, KaiB \xp, KaiC \xp
- J4500\xp from the 97.3 ng/uL midiprep + KaiA \xp, KaiB \xp, KaiC \xp
- J4500\xp from the 97.3 ng/uL midiprep + KaiA \xp, KaiB \xp, KaiC \xp
Did a 1:3 volume ratio because the gel cutout did not give good nanodrop numbers; the usual, 25uL competent cells per sample, plated 5 on KAN (the J4500 \xp), 5 on Carb (the B0034 and the controls), and 1 of the J4500\xp on KAN (the KaiC \xp).
