IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-17: Difference between revisions
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[[2006-8-17_Digest_Gel_Image.jpg]] | {{IGEM:/Harvard/2006/Cyanobacteria}} | ||
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<li>[[IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-16 | 8/16 Entry]]</li> | |||
<li>[[IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-17 | 8/17 Entry]]</li> | |||
<li>[[IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-18 | 8/18 Entry]]</li> | |||
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==Gel image of Kai\X-P, J04500\S-P, J04500\X-P, and B0034\S-P== | |||
[[Image: 2006-8-17_Digest_Gel_Image.jpg|thumb|left|80V for 90 min<br>''Click for Legend'']] | |||
[[Image:2006-08-17 21hr 53min.jpg|thumb|left|80V for 105 min<br>"Click for Legend"]] | |||
==Ligation and Transformation attempt #3 for stage 1 of constructs== | |||
This ligation and transformation attempt was done for 9 things: | |||
*B0034\sp + KaiA \xp, KaiB \xp, KaiC \xp | |||
*J4500\xp from the 97.3 ng/uL midiprep + KaiA \xp, KaiB \xp, KaiC \xp | |||
*J4500\xp from the 97.3 ng/uL midiprep + KaiA \xp, KaiB \xp, KaiC \xp | |||
Did a 1:3 volume ratio because the gel cutout did not give good nanodrop numbers; the usual, 25uL competent cells per sample, plated 5 on KAN (the J4500 \xp), 5 on Carb (the B0034 and the controls), and 1 of the J4500\xp on KAN (the KaiC \xp). |
Latest revision as of 11:58, 21 August 2006
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Gel image of Kai\X-P, J04500\S-P, J04500\X-P, and B0034\S-P
Ligation and Transformation attempt #3 for stage 1 of constructs
This ligation and transformation attempt was done for 9 things:
- B0034\sp + KaiA \xp, KaiB \xp, KaiC \xp
- J4500\xp from the 97.3 ng/uL midiprep + KaiA \xp, KaiB \xp, KaiC \xp
- J4500\xp from the 97.3 ng/uL midiprep + KaiA \xp, KaiB \xp, KaiC \xp
Did a 1:3 volume ratio because the gel cutout did not give good nanodrop numbers; the usual, 25uL competent cells per sample, plated 5 on KAN (the J4500 \xp), 5 on Carb (the B0034 and the controls), and 1 of the J4500\xp on KAN (the KaiC \xp).