IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-18: Difference between revisions
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[[Image: harvard_igem_8_18_blot_g.jpg|thumb|left|The GFP bands]] | [[Image: harvard_igem_8_18_blot_g.jpg|thumb|left|The GFP bands]] | ||
[[Image: harvard_igem_8_18_blot_r.jpg|thumb|left|The film minus the GFP bands]] | [[Image: harvard_igem_8_18_blot_r.jpg|thumb|left|The film minus the GFP bands]] | ||
[[Image: harvard_igem_8_18_blot_merge.jpg | [[Image: harvard_igem_8_18_blot_merge.jpg|thumb|left|Both images together]] | ||
<br style="clear:both"> | |||
==Innoculation of transformation products from yesterday== | |||
Only the: | |||
*4500\xp a\xp | |||
*0034\sp a\xp | |||
*0034\sp c\xp | |||
*4500\xp c\xp | |||
*4500\sp b\xp | |||
*+ cont. | |||
Grew! innoculated 2 clones/ea, except for 0034\sp c\xp. In 4uL with CARB antibiotic. --[[User:Zsun|Zsun]] 20:53, 19 August 2006 (EDT) | |||
==Digest of J04500\xp J04500 \sp KaiABC \xp B0034\sp== | |||
Redigested two of each of the above per. silver protocol ratios, except each enzyme was diluted 3fold. (in other words, each sample had 7.2 uL DNA), 16h @ 45C. The J04500 source was again the 97ng/uL. | |||
Latest revision as of 14:01, 20 August 2006
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Western Blot Images
Today we applied both antibody washes and imaged our GFP Western blot. These are the resulting images:
Innoculation of transformation products from yesterday
Only the:
- 4500\xp a\xp
- 0034\sp a\xp
- 0034\sp c\xp
- 4500\xp c\xp
- 4500\sp b\xp
- + cont.
Grew! innoculated 2 clones/ea, except for 0034\sp c\xp. In 4uL with CARB antibiotic. --Zsun 20:53, 19 August 2006 (EDT)
Digest of J04500\xp J04500 \sp KaiABC \xp B0034\sp
Redigested two of each of the above per. silver protocol ratios, except each enzyme was diluted 3fold. (in other words, each sample had 7.2 uL DNA), 16h @ 45C. The J04500 source was again the 97ng/uL.
