IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-25: Difference between revisions
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I miniprepped the 4 cultures Nick inoculated last night, and labeled them 5-1, 5-2, 8-1, and 8-2. | I miniprepped the 4 cultures Nick inoculated last night, and labeled them 5-1, 5-2, 8-1, and 8-2. | ||
From Nick's email: | From Nick's email: | ||
<code> | |||
All three colonies are KaiB constructs. I have made 4 overnight | All three colonies are KaiB constructs. I have made 4 overnight | ||
cultures with the following labels: | cultures with the following labels: | ||
Revision as of 12:16, 25 August 2006
Miniprep of Nick's cultures #5 and #8
I miniprepped the 4 cultures Nick inoculated last night, and labeled them 5-1, 5-2, 8-1, and 8-2.
From Nick's email:
All three colonies are KaiB constructs. I have made 4 overnight
cultures with the following labels:
"NES 5" is a putative KaiB insert in a J04500 from a 6:1 Insert:Backbone
reaction using the NEB ligase.
"NES 5" is a second culture started from the same colony.
"NES 8" is a putative KaiB insert in a J04500 from a 3:1 Insert:Backbone
reaction using the Roche ligase.
"NES 8" is from a different (*not* clonal) colony from the same ligation
reaction as the other NES 8.
