IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-25

Miniprep of Nick's cultures #5 and #8

I miniprepped the 4 cultures Nick inoculated last night, and labeled them 5-1, 5-2, 8-1, and 8-2.

From Nick's email:

All three colonies are KaiB constructs. I have made 4 overnight cultures with the following labels:

"NES 5" is a putative KaiB insert in a J04500 from a 6:1 Insert:Backbone reaction using the NEB ligase. "NES 5" is a second culture started from the same colony. "NES 8" is a putative KaiB insert in a J04500 from a 3:1 Insert:Backbone reaction using the Roche ligase. "NES 8" is from a different (*not* clonal) colony from the same ligation reaction as the other NES 8.