IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-3

To-do short term

• PCR purify the second batch of digested KaiA/B.
• Gel purify the excised BioBrick backbone (2kb fragment)
  • Ligate the above two products
• Run the second batch of digested RFP BioBrick plasmid on a gel
  • Gel purify the insert
  • (Maybe) Gel purify the backbone
    • Add phosphatase to the backbone to make it ready for ligation
• Prepare our mysterious 400b segment for sequencing