IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-30: Difference between revisions
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==Ligation #6 Plates== | |||
PLATES! | |||
'''Note that for 2uL we got a lot more colonies than for 19uL. Means that the ligation mixture is toxic to the cells; do not use 19uL anymore for chemically competent cells!''' | |||
==Finally== | ==Finally== | ||
It looks like we've finally succeeded in ligating J04500\SP + KaiC\XP! | It looks like we've finally succeeded in ligating J04500\SP + KaiC\XP! | ||
Revision as of 02:38, 30 August 2006
Ligation #6 Plates
PLATES!
Note that for 2uL we got a lot more colonies than for 19uL. Means that the ligation mixture is toxic to the cells; do not use 19uL anymore for chemically competent cells!
Finally
It looks like we've finally succeeded in ligating J04500\SP + KaiC\XP!
