IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-30: Difference between revisions
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[[Image: molar2_830.jpg|thumb|left| Molar Ratio, 2uL ligation product]] | [[Image: molar2_830.jpg|thumb|left| Molar Ratio, 2uL ligation product]] | ||
[[Image: molar19_830.jpg|thumb|right| Molar Ratio, 19uL ligation product]] | [[Image: molar19_830.jpg|thumb|right| Molar Ratio, 19uL ligation product]] | ||
[[Image: volume2_830.jpg|thumb|left| Volume Ratio, 2uL ligation product]] | [[Image: volume2_830.jpg|thumb|left| Volume Ratio, 2uL ligation product]] | ||
[[Image: volume19_830.jpg|thumb|right| Volume Ratio, 19uL ligation product]]<br><br> | [[Image: volume19_830.jpg|thumb|right| Volume Ratio, 19uL ligation product]]<br><br> | ||
Revision as of 02:45, 30 August 2006
Ligation #6 Plates
Thousands of colonies! Excuse the blurriness, used my cameraphone :(
Note that for 2uL we got a lot more colonies than for 19uL. Means that the ligation mixture is toxic to the cells; do not use 19uL anymore for chemically competent cells!
Finally
It looks like we've finally succeeded in ligating J04500\SP + KaiC\XP!
