IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-30
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Ligation #6 Plates
Thousands of colonies! Excuse the blurriness, used my cameraphone :(
Note that for 2uL we got a lot more colonies than for 19uL. Means that the ligation mixture is toxic to the cells; do not use 19uL anymore for chemically competent cells!
Finally
It looks like we've finally succeeded in ligating J04500\SP + KaiC\XP!
