IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-6: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
JeffreyLau (talk | contribs) (→To-do) |
JeffreyLau (talk | contribs) (→To-do) |
||
| Line 4: | Line 4: | ||
# (Maybe) Do an X-P digest of the KaiA+bkb and KaiB+bkb plasmids (though we may want to wait and do this along with the other KaiA+bkb and KaiB+bkb ligation transformants) | # (Maybe) Do an X-P digest of the KaiA+bkb and KaiB+bkb plasmids (though we may want to wait and do this along with the other KaiA+bkb and KaiB+bkb ligation transformants) | ||
# Inoculate the KaiA+bkb and KaiB+bkb transformants from yesterday's ligation | # Inoculate the KaiA+bkb and KaiB+bkb transformants from yesterday's ligation | ||
# Add CIP to the E-S digest of LC vector | # <s>Add CIP to the E-S digest of LC vector</s> | ||
# Run the digests from yesterday on a gel (E-S digest of LC vector, X-P digest of J04450+bkb) | # Run the digests from yesterday on a gel (E-S digest of LC vector, X-P digest of J04450+bkb) | ||
# Gel-extract and purify the LC vector and J04450 insert if they exist | # Gel-extract and purify the LC vector and J04450 insert if they exist | ||
Revision as of 14:07, 6 August 2006
To-do
- Make frozen stocks of the KaiA+bkb and KaiB+bkb transformants that we grew up in liquid culture yesterday
- Miniprep the above
- (Maybe) Do an X-P digest of the KaiA+bkb and KaiB+bkb plasmids (though we may want to wait and do this along with the other KaiA+bkb and KaiB+bkb ligation transformants)
- Inoculate the KaiA+bkb and KaiB+bkb transformants from yesterday's ligation
Add CIP to the E-S digest of LC vector- Run the digests from yesterday on a gel (E-S digest of LC vector, X-P digest of J04450+bkb)
- Gel-extract and purify the LC vector and J04450 insert if they exist
- (maybe) Perform a ligation and transformation of the J04450 insert into LC vector
- Do 12 minipreps for Mingming
KaiA/B + bkb inoculation
We found 9 colonies on the KaiA+bkb plate and 5 colonies on the KaiB+bkb plate.
