IGEM:Harvard/2006/DNA nanostructures

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(create thrombin-aptamer experiments subsection)
(Thrombin-aptamer experiments: bibliography)
Line 28: Line 28:
==Thrombin-aptamer experiments==
==Thrombin-aptamer experiments==
# tha1 pmid=8107090
# tha2 pmid=15945116
# tha3 pmid=8298130
# tha4 pmid=1741036

Revision as of 11:04, 6 July 2006


Project Overview

  • Our goal is to to design and implement molecular containers, which can be dynamically opened and closed by an external stimulus.
  • The containers will be implemented as DNA nanostructures, which afford a significant degree of positional control and chemical versatility.
  • As an initial proof-of-concept, we plan to use our DNA containers to demonstrate controllable activation ("delivery") of anti-thrombin aptamers.
  • We expect that molecular containers could have several interesting scientific and clinical applications, such as
    • Drug and gene delivery
    • Bio-marker scavenging (early detection of biomarkers)
    • Directed evolution (compartmentalized selections)
    • Using multiplexing for combinatorial chemical synthesis
    • Capture and stabilization of multiprotein complexes
    • Protein folding (chaperones)
    • Cell sorting

Container Specs


Container Designs


Existing code

Thrombin-aptamer experiments


  1. Schultze P, Macaya RF, and Feigon J. . pmid:8107090. PubMed HubMed [tha1]
  2. Liu Y, Lin C, Li H, and Yan H. . pmid:15945116. PubMed HubMed [tha2]
  3. Li WX, Kaplan AV, Grant GW, Toole JJ, and Leung LL. . pmid:8298130. PubMed HubMed [tha3]
  4. Bock LC, Griffin LC, Latham JA, Vermaas EH, and Toole JJ. . pmid:1741036. PubMed HubMed [tha4]
All Medline abstracts: PubMed HubMed


Most recent (Week 3)

Week 2: Original proposal

Working Team Members

Personal tools