IGEM:Harvard/2006/DNA nanostructures/Logistics Communications: Difference between revisions
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==Communications== | ==Communications== | ||
===8-23=== | |||
* See top of [[http://openwetware.org/wiki/Table_of_c5.0_Working_Stocks#Working_Stock|c5.0 Table of Working Stocks]]. | |||
* Made up 300mM MgCl2 folding buffer from the HEPES pH 7.35 available in lab from Alain. | |||
===8-18=== | ===8-18=== | ||
Revision as of 17:13, 23 August 2006
Communications
8-23
- See top of [Table of Working Stocks].
- Made up 300mM MgCl2 folding buffer from the HEPES pH 7.35 available in lab from Alain.
8-18
- Needs to be Ordered:
- NEB 1kb ladder
- Needs to be Mixed Up:
- Invitrogen 1kb+ ladder + loading dye solution (see below)
- Changes/Additions in Materials or their Positions:
- Invitrogen 1kb+ ladder stock - in dark green rack (for 50mL -> 1.5mL tubes) on Bench 2
- Invitrogen's manual says use 0.1ug/mm of lane
- In the manual, 0.7ug (ie. 0.7uL) of ladder seems to look good...
- Invitrogen's manual says use 0.1ug/mm of lane
- c5.0.1
- Mixed using 1-10uL multichannel pipettor
- c5.0.4
- c5.0.6
- c5.0.7
- NB: Next time, could whoever finishes the last of a pre-working stock PLEASE make some more, as a courtesy to others?
- c5.0.Eb
- c5.0.Fb
- Proteinase K, reconstituted in 260uL dH2O, as seems to have been indicated by the bottle
- Qiagen seems to have stopped producing this product in this lyophilized form - no instructions on this form were found online
- Invitrogen 1kb+ ladder stock - in dark green rack (for 50mL -> 1.5mL tubes) on Bench 2
