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| weekstart=14 | | weekstart=14 |
| </calendar> | | </calendar> |
|
| |
| ==Thrombin-aptamer experiments==
| |
|
| |
| ====Questions / procedures====
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| * what percent gel? 10% to 20% polyacrylamide gels, no SDS (but would make for a good control)
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| * what incubation conditions?
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| * how much protein and DNA? protein at 1 {{um}}, DNA at 2 {{um}}
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| * Coomassie stain
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|
| |
| ====Experiments====
| |
| {| {{table}}
| |
| | align="center" style="background:#f0f0f0;"|number
| |
| | align="center" style="background:#f0f0f0;"|thrombin
| |
| | align="center" style="background:#f0f0f0;"|aptamer
| |
| | align="center" style="background:#f0f0f0;"|nanotube
| |
| | align="center" style="background:#f0f0f0;"|DNA-stained prediction
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| | align="center" style="background:#f0f0f0;"|protein-stained prediction
| |
| |-
| |
| |0||-||-||-||no bands||no bands
| |
| |-
| |
| |1||-||-||+||slow band (nanotube)||no bands
| |
| |-
| |
| |2||-||+||-||fast band (aptamer)||no bands
| |
| |-
| |
| |3||-||+||+||slow band (aptamer-nanotube), traces of fast band (aptamer)||no bands
| |
| |-
| |
| |4||+||-||-||no bands||fast band (thrombin)
| |
| |-
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| |5||+||-||+||slow band (nanotube)||fast band (thrombin)
| |
| |-
| |
| |6||+||+||-||medium band (aptamer-thrombin), fast band (aptamer)||medium band (aptamer-thrombin), traces of fast band (thrombin)
| |
| |-
| |
| |7||+||+||+||very slow band (thrombin-aptamer-nanotube), slow band (aptamer-nantotube), traces of fast band (aptamer)||very slow band (thrombin-aptamer-nanotube), medium band (aptamer-thrombin), traces of fast band (thrombin)
| |
| |-
| |
| |}
| |
|
| |
| ====Buffers====
| |
| * Macaya's and Bock's selection buffer: 20 mM Tris-acetate, pH 7.4, 140 mM NaCl, 5 mM KCl, 1 mM CaCl<sub>2</sub>, 1 mM MgCl<sub>2</sub>
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| * Liu's incubation buffer: 40 mM Tris, 20 mM CH<sub>3</sub>COOH, 2mM EDTA, 12.5 mM (CH<sub>3</sub>COO)<sub>2</sub>Mg, pH 8.0
| |
| * Liu's PAGE buffer: 1x TAE/Mg<sup>2+</sup>
| |