IGEM:Harvard/2006/DNA nanostructures/Notebook

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m
Line 18: Line 18:
weekstart=14
weekstart=14
</calendar>
</calendar>
-
 
-
==Thrombin-aptamer experiments==
 
-
 
-
====Questions / procedures====
 
-
* what percent gel? 10% to 20% polyacrylamide gels, no SDS (but would make for a good control)
 
-
* what incubation conditions?
 
-
* how much protein and DNA? protein at 1 {{um}}, DNA at 2 {{um}}
 
-
* Coomassie stain
 
-
 
-
====Experiments====
 
-
{| {{table}}
 
-
| align="center" style="background:#f0f0f0;"|number
 
-
| align="center" style="background:#f0f0f0;"|thrombin
 
-
| align="center" style="background:#f0f0f0;"|aptamer
 
-
| align="center" style="background:#f0f0f0;"|nanotube
 
-
| align="center" style="background:#f0f0f0;"|DNA-stained prediction
 
-
| align="center" style="background:#f0f0f0;"|protein-stained prediction
 
-
|-
 
-
|0||-||-||-||no bands||no bands
 
-
|-
 
-
|1||-||-||+||slow band (nanotube)||no bands
 
-
|-
 
-
|2||-||+||-||fast band (aptamer)||no bands
 
-
|-
 
-
|3||-||+||+||slow band (aptamer-nanotube), traces of fast band (aptamer)||no bands
 
-
|-
 
-
|4||+||-||-||no bands||fast band (thrombin)
 
-
|-
 
-
|5||+||-||+||slow band (nanotube)||fast band (thrombin)
 
-
|-
 
-
|6||+||+||-||medium band (aptamer-thrombin), fast band (aptamer)||medium band (aptamer-thrombin), traces of fast band (thrombin)
 
-
|-
 
-
|7||+||+||+||very slow band (thrombin-aptamer-nanotube), slow band (aptamer-nantotube), traces of fast band (aptamer)||very slow band (thrombin-aptamer-nanotube), medium band (aptamer-thrombin), traces of fast band (thrombin)
 
-
|-
 
-
|}
 
-
 
-
====Buffers====
 
-
* Macaya's and Bock's selection buffer: 20 mM Tris-acetate, pH 7.4, 140 mM NaCl, 5 mM KCl, 1 mM CaCl<sub>2</sub>, 1 mM MgCl<sub>2</sub>
 
-
* Liu's incubation buffer: 40 mM Tris, 20 mM CH<sub>3</sub>COOH, 2mM EDTA, 12.5 mM (CH<sub>3</sub>COO)<sub>2</sub>Mg, pH 8.0
 
-
* Liu's PAGE buffer: 1x TAE/Mg<sup>2+</sup>
 

Revision as of 16:47, 11 July 2006


June
SMTWTFS
45678910
11121314151617
18192021222324
252627282930
July
SMTWTFS
2345678
9101112131415
16171819202122
23242526272829
3031
August
SMTWTFS
6789101112
13141516171819
20212223242526
2728293031
Personal tools