IGEM:Harvard/2006/DNA nanostructures/Notebook: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
mNo edit summary
mNo edit summary
Line 1: Line 1:
{{IGEM H06 DNA nano navbar}}
<div class="tabs-blue">
<ul>
<li>[[IGEM:Harvard/2006/DNA nanostructures|Project Overview]]</li>
<li>[[IGEM:Harvard/2006/DNA_nanostructures/Designs|Designs]]</li>
<li id="current">[[IGEM:Harvard/2006/DNA_nanostructures/Notebook|Notebook]]</li>
<li>[[IGEM:Harvard/2006/DNA_nanostructures/Protocols|Protocols]]</li>
<li>[[IGEM:Harvard/2006/DNA_nanostructures/Presentations|Presentations]]</li>
<li>[[IGEM:Harvard/2006/DNA_nanostructures/Literature|Literature]]</li>
</ul>
</div>
<br style="clear:both">


<calendar>
<calendar>

Revision as of 12:18, 11 July 2006


<calendar> name=IGEM:Harvard/2006/DNA_nanostructures/Notebook date=2006/07/01 view=threemonths format=%name/%year-%month-%day weekstart=14 </calendar>

Thrombin-aptamer experiments

Questions / procedures

  • what percent gel? 10% to 20% polyacrylamide gels, no SDS (but would make for a good control)
  • what incubation conditions?
  • how much protein and DNA? protein at 1 μM, DNA at 2 μM
  • Coomassie stain

Experiments

number thrombin aptamer nanotube DNA-stained prediction protein-stained prediction
0 - - - no bands no bands
1 - - + slow band (nanotube) no bands
2 - + - fast band (aptamer) no bands
3 - + + slow band (aptamer-nanotube), traces of fast band (aptamer) no bands
4 + - - no bands fast band (thrombin)
5 + - + slow band (nanotube) fast band (thrombin)
6 + + - medium band (aptamer-thrombin), fast band (aptamer) medium band (aptamer-thrombin), traces of fast band (thrombin)
7 + + + very slow band (thrombin-aptamer-nanotube), slow band (aptamer-nantotube), traces of fast band (aptamer) very slow band (thrombin-aptamer-nanotube), medium band (aptamer-thrombin), traces of fast band (thrombin)

Buffers

  • Macaya's and Bock's selection buffer: 20 mM Tris-acetate, pH 7.4, 140 mM NaCl, 5 mM KCl, 1 mM CaCl2, 1 mM MgCl2
  • Liu's incubation buffer: 40 mM Tris, 20 mM CH3COOH, 2mM EDTA, 12.5 mM (CH3COO)2Mg, pH 8.0
  • Liu's PAGE buffer: 1x TAE/Mg2+
Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Harvard/2006/DNA_nanostructures/Notebook&oldid=48943"