IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-11-17: Difference between revisions
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==Gel Analysis== | ==Gel Analysis== | ||
- samples: c5.0A (no lids, no aptamers), c5.0C (no lids, inside aptamers), c5.0D (no lids, outside aptamers) | - samples: c5.0A (no lids, no aptamers), c5.0C (no lids, inside aptamers), c5.0D (no lids, outside aptamers) | ||
- 2.0% agarose gel: 2 g agarose + 100 {{ul}} 0.5x TBE + 1.1 mL 1 M {{ | |||
- lanes: 1 = ladder (10 {{ul}}) | - 2.0% agarose gel: 2 g agarose + 100 {{ul}} 0.5x TBE + 1.1 mL 1 M {{mgcl2}} + 5 {{ul}} EtBr | ||
- lanes: | |||
1 = ladder (10 {{ul}}) | |||
2 = p7308 scaffold (5 {{ul}} + 3 {{ul}} dye) | 2 = p7308 scaffold (5 {{ul}} + 3 {{ul}} dye) | ||
3 = c5.0A (5 {{ul}} + 3 {{ul}} dye) | 3 = c5.0A (5 {{ul}} + 3 {{ul}} dye) | ||
4 = c5.0C (5 {{ul}} + 3 {{ul}} dye) | 4 = c5.0C (5 {{ul}} + 3 {{ul}} dye) | ||
5 = c5.0D (5 {{ul}} + 3 {{ul}} dye) | 5 = c5.0D (5 {{ul}} + 3 {{ul}} dye) | ||
- accidentally ran the gel backwards (repeat on Mon.) | |||
Latest revision as of 09:34, 20 November 2006
Gel Analysis
- samples: c5.0A (no lids, no aptamers), c5.0C (no lids, inside aptamers), c5.0D (no lids, outside aptamers)
- 2.0% agarose gel: 2 g agarose + 100 μL 0.5x TBE + 1.1 mL 1 M MgCl2 + 5 μL EtBr
- lanes:
1 = ladder (10 μL)
2 = p7308 scaffold (5 μL + 3 μL dye)
3 = c5.0A (5 μL + 3 μL dye)
4 = c5.0C (5 μL + 3 μL dye)
5 = c5.0D (5 μL + 3 μL dye)
- accidentally ran the gel backwards (repeat on Mon.)
