IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-11-17

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(Gel Analysis)
(Gel Analysis)
Line 4: Line 4:
- 2.0% agarose gel: 2 g agarose + 100 {{ul}} 0.5x TBE + 1.1 mL 1 M {{mgcl2}} + 5 {{ul}} EtBr
- 2.0% agarose gel: 2 g agarose + 100 {{ul}} 0.5x TBE + 1.1 mL 1 M {{mgcl2}} + 5 {{ul}} EtBr
-
- lanes: 1 = ladder (10 {{ul}})
+
- lanes:  
 +
            1 = ladder (10 {{ul}})
             2 = p7308 scaffold (5 {{ul}} + 3 {{ul}} dye)
             2 = p7308 scaffold (5 {{ul}} + 3 {{ul}} dye)
             3 = c5.0A (5 {{ul}} + 3 {{ul}} dye)
             3 = c5.0A (5 {{ul}} + 3 {{ul}} dye)
             4 = c5.0C (5 {{ul}} + 3 {{ul}} dye)
             4 = c5.0C (5 {{ul}} + 3 {{ul}} dye)
             5 = c5.0D (5 {{ul}} + 3 {{ul}} dye)
             5 = c5.0D (5 {{ul}} + 3 {{ul}} dye)

Revision as of 12:28, 17 November 2006

Gel Analysis

- samples: c5.0A (no lids, no aptamers), c5.0C (no lids, inside aptamers), c5.0D (no lids, outside aptamers)

- 2.0% agarose gel: 2 g agarose + 100 μL 0.5x TBE + 1.1 mL 1 M MgCl2 + 5 μL EtBr

- lanes:

            1 = ladder (10 μL)
            2 = p7308 scaffold (5 μL + 3 μL dye)
            3 = c5.0A (5 μL + 3 μL dye)
            4 = c5.0C (5 μL + 3 μL dye)
            5 = c5.0D (5 μL + 3 μL dye)
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