IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-11-20
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< IGEM:Harvard | 2006 | DNA nanostructures | Notebook(Difference between revisions)
(→Gel Analysis (repeat)) |
Current revision (15:45, 27 November 2006) (view source) (→Gel Analysis (repeat)) |
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| + | - results: the folding did not succeed, as can be seen by the lack of bands in the 3rd through 5th lanes near the position of the scaffold | ||
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Current revision
Gel Analysis (repeat)
- samples: c5.0A (no lids, no aptamers), c5.0C (no lids, inside aptamers), c5.0D (no lids, outside aptamers)
- 2.0% agarose gel: 2 g agarose + 100 μL 0.5x TBE + 1.1 mL 1 M MgCl2 + 5 μL EtBr
- lanes:
1 = ladder (10 μL)
2 = p7308 scaffold (5 μL + 3 μL dye)
3 = c5.0A (5 μL + 3 μL dye)
4 = c5.0C (5 μL + 3 μL dye)
5 = c5.0D (5 μL + 3 μL dye)
- run 1 hr. @ 130 V
- results: the folding did not succeed, as can be seen by the lack of bands in the 3rd through 5th lanes near the position of the scaffold
band
