IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-11-30

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Current revision (15:07, 5 December 2006) (view source)
 
Line 1: Line 1:
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8% PEG:
+
===8% PEG===
- 20 {{ul}} 20% PEG + 5 {{ul}} nanostructures + 5 {{ul}} NaCl
- 20 {{ul}} 20% PEG + 5 {{ul}} nanostructures + 5 {{ul}} NaCl
 +
- put on ice 15 min.
- put on ice 15 min.
 +
- spin @ 16k rcf for 10 min.
- spin @ 16k rcf for 10 min.
 +
- pipette out supernatant
- pipette out supernatant
 +
- resuspend pellet in 10x folding buffer
- resuspend pellet in 10x folding buffer

Current revision

8% PEG

- 20 μL 20% PEG + 5 μL nanostructures + 5 μL NaCl


- put on ice 15 min.

- spin @ 16k rcf for 10 min.

- pipette out supernatant

- resuspend pellet in 10x folding buffer

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