IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-7-12: Difference between revisions

Revision as of 07:36, 12 July 2006

Results from 7/11 Thrombin-Binding Experiment

gels removed at 10:00 am: appear to have run off the gel (very hard to tell since they were so faint to begin with)
removed gels from plastic
- cut bottom of gel where it sticks out of narrow hole at the bottom
- pried two plastic sides apart (gel now stuck to one side)
- loosen top edge by sliding flat tool under it a bit.
- make sure the very bottom is completely cut
- run it under water over the a plastic tupperware container - move it back and forth left to right - eventually the gel should come free into the container.
- hold the gel in the container while you pour the water out.
stained with Coomassie blue (in brown bottle in first 4degree fridge)
- pour enough coomassie blue over it to cover the gel.
- put the lid on the tupperware and put it on the rocker for about 20 minutes.

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 Results from 7/11 Thrombin-Binding Experiment
-* gels removed at 10:00 am: appear to have run off the gel
+* gels removed at 10:00 am: appear to have run off the gel (very hard to tell since they were so faint to begin with)
+* removed gels from plastic
+** cut bottom of gel where it sticks out of narrow hole at the bottom
+** pried two plastic sides apart (gel now stuck to one side)
+** loosen top edge by sliding flat tool under it a bit.
+** make sure the very bottom is completely cut
+** run it under water over the a plastic tupperware container - move it back and forth left to right - eventually the gel should come free into the container.
+** hold the gel in the container while you pour the water out.
+* stained with Coomassie blue (in brown bottle in first 4degree fridge)
+** pour enough coomassie blue over it to cover the gel.
+** put the lid on the tupperware and put it on the rocker for about 20 minutes.