IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-7-13
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Initial Container v3.2 Preparation and Analysis
- Oligos arrived this morning from Invitrogen. Plan for today is to mix oligos together into pre-stocks, and then working stocks, and then try some initial folding experiments and gel analysis.
Creating pre-working stocks and working stocks from design 3.2 oligos
- Mix 10 μL of each 50 μM stock oligo
| oligo | desc | plate location | total |
| c3.2.1 | core barrel | 3.2p1A01–3.2p1H10 | 94 |
| c3.2.2 | core top lid | 3.2p2A01–3.2p2C04 | 28 |
| c3.2.3 | core bottom lid | 3.2p2C05–3.2p2E10 | 30 |
| c3.2.4 | barrel at inside apatamer locations -aptamers | 3.2p2E11–3.2p2F01 | 3 |
| c3.2.5 | barrel at outside aptamer locations -aptamers | 3.2p2F02–3.2p2F04 | 3 |
| c3.2.6 | barrel at inside apatamer locations +aptamers | 3.2p2F05–3.2p2F07 | 3 |
| c3.2.7 | barrel at outside aptamer locations +aptamers | 3.2p2F08–3.2p2F10 | 3 |
| c3.2.8 | barrel at latch locations -latches | 3.2p2F11–3.2p2F12 | 2 |
| c3.2.9 | lid at latch locations -latches (empty) | 0 | |
| c3.2.10 | barrel at latch locations +latch1 +latch2 | 3.2p2G01–3.2p2G02 | 2 |
| c3.2.11 | latch from barrel +latch1 -latch2 | 3.2p2G03–3.2p2G04 | 2 |
| c3.2.12 | latch from barrel -latch1 +latch2 | 3.2p2G05–3.2p2G06 | 2 |
| c3.2.13 | lid at latch locations +latch1 +latch2 (empty) | 0 | |
| c3.2.14 | latch from lids +latch1 -latch2 | 3.2p2G07–3.2p2G08 | 2 |
| c3.2.15 | latch from lids -latch1 +latch2 | 3.2p2G09–3.2p2G10 | 2 |
| c3.2.16 | latch staples +latch2 | 3.2p2G11–3.2p2H02 | 4 |
| c3.2.17 | displacement strands latch1 | 3.2p2H03–3.2p2H06 | 4 |
| c3.2.18 | displacement strands latch2 | 3.2p2H07–3.2p2H10 | 4 |
