IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-7-16: Difference between revisions
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(→c3.2 Folding Experiment: 40 ul) |
(→c3.2 Folding Experiment: 6 hb control) |
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| C||9 {{ul}} p7308||16 {{ul}} 3.2.C||4 {{ul}} 10x||11 {{ul}}||40 {{ul}} | | C||9 {{ul}} p7308||16 {{ul}} 3.2.C||4 {{ul}} 10x||11 {{ul}}||40 {{ul}} | ||
|- | |||
| 6hb||4.5 {{ul}} p7308||2 {{ul}} 6hb.v5 (0.99 {{um}})||2 {{ul}} 10x||11.5 {{ul}}||20 {{ul}} | |||
|} | |} | ||
Annealing protocol | Annealing protocol | ||
Revision as of 23:13, 15 July 2006
Container v3.2
c3.2 Folding Experiment
Reagents (each expt in respective 0.2 mL PCR tube)
| Experiment | Scaffold | Oligos | Folding buffer | dH2O | total volume |
| B | 9 μL p7308 | 16 μL 3.2.B | 4 μL 10x | 11 μL | 40 μL |
| C | 9 μL p7308 | 16 μL 3.2.C | 4 μL 10x | 11 μL | 40 μL |
| 6hb | 4.5 μL p7308 | 2 μL 6hb.v5 (0.99 μM) | 2 μL 10x | 11.5 μL | 20 μL |
Annealing protocol
- start at 80[[:Category:{{{1}}}|{{{1}}}]]
- 60 cycles: wait 2 minutes, decrease 1[[:Category:{{{1}}}|{{{1}}}]]
- hold at 4[[:Category:{{{1}}}|{{{1}}}]]
