IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-7-16
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Container v3.2
c3.2 Folding Experiment
Reagents (each expt in respective 0.2 mL PCR tube)
Experiment | Scaffold | Oligos | Folding buffer | dH2O | total volume |
B | 4.5 μL p7308 | 8 μL 3.2.B | 2 μL 10x | 5.5 μL | 20 μL |
C | 4.5 μL p7308 | 8 μL 3.2.C | 2 μL 10x | 5.5 μL | 20 μL |
Annealing protocol
- start at 80[[:Category:{{{1}}}|{{{1}}}]]
- 60 cycles: wait 2 minutes, decrease 1[[:Category:{{{1}}}|{{{1}}}]]
- hold at 4[[:Category:{{{1}}}|{{{1}}}]]