IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-7-18: Difference between revisions
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'''Protection assay''' | '''Protection assay''' | ||
==Protection assay== | |||
'''Questions''' | |||
*Can we digest streptavidin with [http://www1.qiagen.com/Products/Accessories/AccessoryEnzymes/QIAGENProteinaseK.aspx Qiagen proteinase K] and/or [http://www1.qiagen.com/Products/Accessories/AccessoryEnzymes/QIAGENProtease.aspx Qiagen protease]? | *Can we digest streptavidin with [http://www1.qiagen.com/Products/Accessories/AccessoryEnzymes/QIAGENProteinaseK.aspx Qiagen proteinase K] and/or [http://www1.qiagen.com/Products/Accessories/AccessoryEnzymes/QIAGENProtease.aspx Qiagen protease]? | ||
*What alternative proteases can we use? | *What alternative proteases can we use? | ||
** We're going to try trypsin | |||
*What is our initial protocol? | *What is our initial protocol? | ||
Revision as of 09:08, 18 July 2006
Questions
Design 2
- How big does a 2-ply lid need to be to span a 30-helix barrel?
- Can we use two scaffolds in the design?
- 2x42bp repeats in barrel
- 30-helix bundle with 30*(2*42+12) repeats = 2880 bases
- two-ply-lid: 36 * (2*42+12) = 3456 * 2 = 6912
- using single scaffold for 2880-base 30hb: 9792 bases total = $783.36
- 3x42bp repeats in barrel
- 30-helix bundle with 30*(3*42+12) repeats = 4140 bases
- two-ply-lid: 36 * (2*42+12) = 3456 * 2 = 6912
- using single scaffold for 4140-base 30hb: 11052 bases total = $884.16
- Will unused scaffold interfere with proper folding of lid & barrels
- Should we use two M13-based scaffolds, or try to make ΦX174 to have less slack?
- Can we easily get ΦX174? fermentas
Protection assay
Protection assay
Questions
- Can we digest streptavidin with Qiagen proteinase K and/or Qiagen protease?
- What alternative proteases can we use?
- We're going to try trypsin
- What is our initial protocol?
