IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-7-24: Difference between revisions

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| 6||1.0||-||4.0 (same b-working stock)||-||8.0||2
| 6||1.0||-||4.0 (same b-working stock)||-||8.0||2
|-
|-
| 7||1.0||-||-||10.0** (no strep binding site)||2.0||2
| 7||1.0||-||-||10.0** (3.2.A)||2.0||2
|-
|-
| 8||1.0||-||-||10.0** (outward strep binding site)||2.0||2
| 8||1.0||-||-||10.0** (3.2.Eb or 4.0 Ib||2.0||2
|-
|-
| 9||1.0||-||-||10.0** (outward strep binding site w/ latch)||2.0||2
| 9||1.0||-||-||10.0** (3.2.Ib or 4.0.Ib)||2.0||2
|-
|-
| 10||1.0||-||-||10.0** (inward strep binding site)||2.0||2
| 10||1.0||-||-||10.0** (3.2.Db or 4.0.Db)||2.0||2
|-
|-
| 11||1.0||-||-||10.0** (inward strep binding site w/ latch)||2.0||2
| 11||1.0||-||-||10.0** (3.2.Hb or 4.0.Hb)||2.0||2
|-
|-
| 12||1.0||-||-||10.0** (inward and outward strep binding sites) ||2.0||2
| 12||1.0||-||-||10.0** (3.2.Lb) ||2.0||2
|}
|}

Revision as of 14:34, 24 July 2006

Precipitation tests

Streptavidin binding assay

  • Double asterisks indicate need for purified nanostructures
  • Total volume for each reaction: 13 μL
lane streptavidin (0.5 μM) (μL) p7308 scaffold (44 nM) (μL) oligos (250 nM) (μL) nanostructures (10 nM) (μL) water (μL) 10x Tris-gly loading dye (μL)
2 1.0 - - - 12.0 2
3 - 11.4 - - 1.6 2
4 1.0 11.4 - - 0.6 2
5 - - 4.0 (a b-working stock) - 9.0 2
6 1.0 - 4.0 (same b-working stock) - 8.0 2
7 1.0 - - 10.0** (3.2.A) 2.0 2
8 1.0 - - 10.0** (3.2.Eb or 4.0 Ib 2.0 2
9 1.0 - - 10.0** (3.2.Ib or 4.0.Ib) 2.0 2
10 1.0 - - 10.0** (3.2.Db or 4.0.Db) 2.0 2
11 1.0 - - 10.0** (3.2.Hb or 4.0.Hb) 2.0 2
12 1.0 - - 10.0** (3.2.Lb) 2.0 2
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