IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-7-31: Difference between revisions
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==Redesigning the DNA ligand== | ==Redesigning the DNA ligand== | ||
It turns out that NotI requires 10bp on each side of the restriction site for succesful digests. We need to redesign the [[IGEM:Harvard/2006/DNA_nanostructures/Notebook/2006-7-26#Oligo_design|old DNA ligand]]. | |||
Other possible restriction enzymes (using [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp NEB cleavage experiments] to determine minimum number of bp needed on each side): | Other possible restriction enzymes (using [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp NEB cleavage experiments] to determine minimum number of bp needed on each side): | ||
Revision as of 10:18, 31 July 2006
Redesigning the DNA ligand
It turns out that NotI requires 10bp on each side of the restriction site for succesful digests. We need to redesign the old DNA ligand.
Other possible restriction enzymes (using NEB cleavage experiments to determine minimum number of bp needed on each side):
- AflIII (ACATGT)
- sequence not present in p7308
- >90% yield after 2 hr digest with 2 bp on each side
- AscIII (GGCGCGCC)
- sequence not present in p7308
- >90% yield after 2 hr digest with 0 bp on each side
- StuI (AGGCCT)
- sequence not present in p7308
- >90% yield after 2 hr digest with 1 bp on each side
