IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-14: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Goals for today== | ==Goals for today== | ||
Purification | |||
===Microcon Purification Tweaking=== | |||
* repeat Friday's mega PEG ppt on 5.0 (?) | * repeat Friday's mega PEG ppt on 5.0 (?) | ||
* Micron experiments with 0.1% and 0.01% SDS in buffer | * Micron experiments with 0.1% and 0.01% SDS in buffer | ||
** ...and use 1x folding buffer and not water for washes | ** ...and use 1x folding buffer and not water for washes | ||
** also: perform control expt with 10 bp+ ladder, since according to [http://www.millipore.com/publications.nsf/docs/6dkp6d Millipore documentation], the filter should retain ds DNAs longer than 100 bp | ** also: perform control expt with 10 bp+ ladder, since according to [http://www.millipore.com/publications.nsf/docs/6dkp6d Millipore documentation], the filter should retain ds DNAs longer than 100 bp | ||
===Streptavidin-Bead "Protection" Assay on Inside- and Outside-Biotinylated c5.0=== | |||
*NB: no good purification of nanostructure from oligo has been achieved, but gel separation after elution should differentiate formerly bead-bound oligos from formerly bead-bound nanostructures | |||
===Redux of [Mg++], [oligos]=== | |||
* Based on [[IGEM:Harvard/2006/DNA_nanostructures/Notebook/2006-8-7|the inconclusive gels from last Monday]], the titration will be redone with c5.0 at 8% PEG, 0.5M NaCl '''final'''. | |||
==Microcon w/ detergent== | ==Microcon w/ detergent== | ||
Revision as of 10:42, 14 August 2006
Goals for today
Microcon Purification Tweaking
- repeat Friday's mega PEG ppt on 5.0 (?)
- Micron experiments with 0.1% and 0.01% SDS in buffer
- ...and use 1x folding buffer and not water for washes
- also: perform control expt with 10 bp+ ladder, since according to Millipore documentation, the filter should retain ds DNAs longer than 100 bp
Streptavidin-Bead "Protection" Assay on Inside- and Outside-Biotinylated c5.0
- NB: no good purification of nanostructure from oligo has been achieved, but gel separation after elution should differentiate formerly bead-bound oligos from formerly bead-bound nanostructures
Redux of [Mg++], [oligos]
- Based on the inconclusive gels from last Monday, the titration will be redone with c5.0 at 8% PEG, 0.5M NaCl final.
