IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-14

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Revision as of 13:37, 14 August 2006

Goals for today

Purification.

  • repeat Friday's mega PEG ppt on 5.0 (?)
  • Micron experiments with 0.1% and 0.01% SDS in buffer
    • ...and use 1x folding buffer and not water for washes
    • also: perform control expt with 10 bp+ ladder, since according to Millipore documentation, the filter should retain ds DNAs longer than 100 bp

Microcon w/ detergent

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