IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-14

Goals for today

Microcon Purification Tweaking

• repeat Friday's mega PEG ppt on 5.0 (?)
• Micron experiments with 0.1% and 0.01% SDS in buffer
  • ...and use 1x folding buffer and not water for washes
  • also: perform control expt with 10 bp+ ladder, since according to Millipore documentation, the filter should retain ds DNAs longer than 100 bp

Streptavidin-Bead "Protection" Assay on Inside- and Outside-Biotinylated c5.0

• NB: no good purification of nanostructure from oligo has been achieved, but gel separation after elution should differentiate formerly bead-bound oligos from formerly bead-bound nanostructures

Redux of [Mg++], [oligos]

• Based on the inconclusive gels from last Monday, the titration will be redone with c5.0 at 8% PEG, 0.5M NaCl final.