IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-15: Difference between revisions
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(→3. PEG) |
(→3. PEG) |
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* resuspended "pellet" in 50 {{ul}} of water | * resuspended "pellet" in 50 {{ul}} of water | ||
[[Image:Igemharv06_PEGprecipitation_gel_1a.jpg | [[Image:Igemharv06_PEGprecipitation_gel_1a.jpg|Gel 1 after 45 minutes]] | ||
[[Image:Igemharv06_PEGprecipitation_gel_2a.jpg | [[Image:Igemharv06_PEGprecipitation_gel_2a.jpg|Gel 2 after 45 minutes]] | ||
[[Image:Igemharv06_PEGprecipitation_gel_1b.jpg | [[Image:Igemharv06_PEGprecipitation_gel_1b.jpg|Gel 1 after 75 minutes]] | ||
[[Image:Igemharv06_PEGprecipitation_gel_2b.jpg | [[Image:Igemharv06_PEGprecipitation_gel_2b.jpg|Gel 2 after 75 minutes]] | ||
Revision as of 12:10, 15 August 2006
Mg2+, Oligo-Concentration Titration w/ c5.0 (cont'd)
3. PEG
PEG:
- GOAL: test 6%, 8%, 10% PEG precipitations
- incubated on ice for 15 min.
- spun at 16 k rcf at 4[[:Category:{{{1}}}|{{{1}}}]] for 10 min.
- carefully pipetted off supernatant
- resuspended "pellet" in 50 μL of water
| Trial | Final PEG % | Lanes | 20% PEG (μL) | 5 M NaCl (μL) | Nanostructures (μL) | Water (μL) | Total volume (μL) |
| 1kb+ ladder | - | 1 | - | - | - | - | 10 |
| p7308 | - | 2 | - | - | - | - | 10 |
| 1 | 0% | 3 (untreated trial 1) | - | - | - | - | - |
| 1-6 | 6% | 4 (pellet) | 15 | 5 | 20 | 10 | 50 |
| 1-6 | 6% | 5 (supernatant) | - | - | - | - | - |
| 1-8 | 8% | 6 (pellet) | 20 | 5 | 20 | 5 | 50 |
| 1-8 | 8% | 7 (supernatant) | - | - | - | - | - |
| 1-10 | 10% | 8 (pellet) | 25 | 5 | 20 | 0 | 50 |
| 1-10 | 10% | 9 (supernatant) | - | - | - | - | - |
| 2 | 0% | 10 (untreated trial 2) | - | - | - | - | - |
| 2-6 | 6% | 11 (pellet) | 15 | 5 | 20 | 10 | 50 |
| 2-6 | 6% | 12 (supernatant) | - | - | - | - | - |
| 2-8 | 8% | 13 (pellet) | 20 | 5 | 20 | 5 | 50 |
| 2-8 | 8% | 14 (supernatant) | - | - | - | - | - |
| 2-10 | 10% | 15 (pellet) | 25 | 5 | 20 | 0 | 50 |
| 2-10 | 10% | 16 (supernatant) | - | - | - | - | - |
| 3 | 0% | 17 (untreated trial 3) | - | - | - | - | - |
| 3-6 | 6% | 18 (pellet) | 15 | 5 | 20 | 10 | 50 |
| 3-6 | 6% | 19 (supernatant) | - | - | - | - | - |
| 1kb+ ladder | - | 1 | - | - | - | - | 10 |
| p7308 | - | 2 | - | - | - | - | 10 |
| 3-8 | 8% | 3 (pellet) | 20 | 5 | 20 | 5 | 50 |
| 3-8 | 8% | 4 (supernatant) | - | - | - | - | - |
| 3-10 | 10% | 5 (pellet) | 25 | 5 | 20 | 0 | 50 |
| 3-10 | 10% | 6 (supernatant) | - | - | - | - | - |
| 4 | 0% | 7 (untreated trial 4) | - | - | - | - | - |
| 4-6 | 6% | 8 (pellet) | 15 | 5 | 20 | 10 | 50 |
| 4-6 | 6% | 9 (supernatant) | - | - | - | - | - |
| 4-8 | 8% | 10 (pellet) | 20 | 5 | 20 | 5 | 50 |
| 4-8 | 8% | 11 (supernatant) | - | - | - | - | - |
| 4-10 | 10% | 12 (pellet) | 25 | 5 | 20 | 0 | 50 |
| 4-10 | 10% | 13 (supernatant) | - | - | - | - | - |
| 5 | 0% | 14 (untreated trial 5) | - | - | - | - | - |
| 5-6 | 6% | 15 (pellet) | 15 | 5 | 20 | 10 | 50 |
| 5-6 | 6% | 16 (supernatant) | - | - | - | - | - |
| 5-8 | 8% | 17 (pellet) | 20 | 5 | 20 | 5 | 50 |
| 5-8 | 8% | 18 (supernatant) | - | - | - | - | - |
| 5-10 | 10% | 19 (pellet) | 25 | 5 | 20 | 0 | 50 |
| 5-10 | 10% | 20 (supernatant) | - | - | - | - | - |
| 1kb+ ladder | - | 1 | - | - | - | - | 10 |
| p7308 | - | 2 | - | - | - | - | 10 |
| 6 | 0% | 3 (untreated trial 6) | - | - | - | - | |
| 6-6 | 6% | 4 (pellet) | 15 | 5 | 20 | 10 | 50 |
| 6-6 | 6% | 5 (supernatant) | - | - | - | - | - |
| 6-8 | 8% | 6 (pellet) | 20 | 5 | 20 | 5 | 50 |
| 6-8 | 8% | 7 (supernatant) | - | - | - | - | - |
| 6-10 | 10% | 8 (pellet) | 25 | 5 | 20 | 0 | 50 |
| 6-10 | 10% | 9 (supernatant) | - | - | - | - | - |
| 6hb-4 | 4% | 10 (pellet) | 10 | 5 | 20 | 15 | 50 |
| 6hb-4 | 4% | 11 (supernatant) | - | - | - | - | - |
| 6hb-6 | 6% | 12 (pellet) | 15 | 5 | 20 | 10 | 50 |
| 6hb-6 | 6% | 13 (supernatant) | - | - | - | - | - |
| 6hb-8 | 8% | 14 (pellet) | 20 | 5 | 20 | 5 | 50 |
| 6hb-8 | 8% | 15 (supernatant) | - | - | - | - | - |
| 6hb-10 | 10% | 16 (pellet) | 25 | 5 | 20 | 0 | 50 |
| 6hb-10 | 10% | 17 (supernatant) | - | - | - | - | - |
