IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-15

Mg2+, Oligo-Concentration Titration w/ c5.0 (cont'd)

3. PEG

PEG:

• GOAL: test 6%, 8%, 10% PEG precipitations
• incubated on ice for 15 min.
• spun at 16 k rcf at 4[[:Category:{{{1}}}|{{{1}}}]] for 10 min.
• carefully pipetted off supernatant
• resuspended "pellet" in 50 μL of water

Trial	Final PEG %	Lanes	20% PEG (μL)	5 M NaCl (μL)	Nanostructures (μL)	Water (μL)	Total volume (μL)
1-6	6%	1 (pellet)	15	5	20	10	50
1-6	6%	2 (supernatant)	-	-	-	-	-
1-8	8%	3 (pellet)	20	5	20	5	50
1-8	8%	4 (supernatant)	-	-	-	-	-
1-10	10%	5 (pellet)	25	5	20	0	50
1-10	10%	6 (supernatant)	-	-	-	-	-
2-6	6%	7 (pellet)	15	5	20	10	50
2-6	6%	8 (supernatant)	-	-	-	-	-
2-8	8%	9 (pellet)	20	5	20	5	50
2-8	8%	10 (supernatant)	-	-	-	-	-
1kb+ ladder	-	11	-	-	-	-	10
p7308	-	12	-	-	-	-	10
3-6	6%	11 (pellet)	15	5	20	10	50
3-6	6%	12 (supernatant)	-	-	-	-	-
3-8	8%	13 (pellet)	20	5	20	5	50
3-8	8%	14 (supernatant)	-	-	-	-	-
3-10	10%	15 (pellet)	25	5	20	0	50
3-10	10%	16 (supernatant)	-	-	-	-	-
4-6	6%	17 (pellet)	15	5	20	10	50
4-6	6%	18 (supernatant)	-	-	-	-	-
4-8	8%	19 (pellet)	20	5	20	5	50
4-8	8%	20 (supernatant)	-	-	-	-	-
4-10	10%	1 (pellet)	25	5	20	0	50
4-10	10%	2 (supernatant)	-	-	-	-	-
5-6	6%	3 (pellet)	15	5	20	10	50
5-6	6%	4 (supernatant)	-	-	-	-	-
5-8	8%	5 (pellet)	20	5	20	5	50
5-8	8%	6 (supernatant)	-	-	-	-	-
5-10	10%	7 (pellet)	25	5	20	0	50
5-10	10%	8 (supernatant)	-	-	-	-	-
6-6	6%	9 (pellet)	15	5	20	10	50
6-6	6%	10 (supernatant)	-	-	-	-	-
1kb+ ladder	-	11	-	-	-	-	10
p7308	-	12	-	-	-	-	10
6-8	8%	13 (pellet)	20	5	20	5	50
6-8	8%	14 (supernatant)	-	-	-	-	-
6-10	10%	15 (pellet)	25	5	20	0	50
6-10	10%	16 (supernatant)	-	-	-	-	-