IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-16

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(Repeat PEG Precipitation + Protection Assay)
(Overview)
Line 3: Line 3:
===Overview===
===Overview===
* concentrate on 30 mM {{mgcl2}} folding buffer, 1x oligos trial from yesterday (yielded best PEG results)
* concentrate on 30 mM {{mgcl2}} folding buffer, 1x oligos trial from yesterday (yielded best PEG results)
-
* folding rxns: 32 (40 {{ul}} rxn) c5.0C (no latch, inside ligand)
+
* folding rxns:  
-
                      32 (40 {{ul}} rxn) c5.0D (no latch, outside ligand)
+
    32 (40 {{ul}} rxn) c5.0C (no latch, inside ligand)
-
                      8 (40 {{ul}} rxn) c5.0A (no latch, no ligand)
+
    32 (40 {{ul}} rxn) c5.0D (no latch, outside ligand)
 +
    8 (40 {{ul}} rxn) c5.0A (no latch, no ligand)
* repeat 8%, 10% PEG precipitation
* repeat 8%, 10% PEG precipitation

Revision as of 10:48, 16 August 2006

Repeat PEG Precipitation + Protection Assay

Overview

  • concentrate on 30 mM MgCl2 folding buffer, 1x oligos trial from yesterday (yielded best PEG results)
  • folding rxns:
    32 (40 μL rxn) c5.0C (no latch, inside ligand)
    32 (40 μL rxn) c5.0D (no latch, outside ligand)
    8 (40 μL rxn) c5.0A (no latch, no ligand)
  • repeat 8%, 10% PEG precipitation
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