IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-16
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===Overview=== | ===Overview=== | ||
* concentrate on 30 mM {{mgcl2}} folding buffer, 1x oligos trial from yesterday (yielded best PEG results) | * concentrate on 30 mM {{mgcl2}} folding buffer, 1x oligos trial from yesterday (yielded best PEG results) | ||
| - | * folding rxns: 32 (40 {{ul}} rxn) c5.0C (no latch, inside ligand) | + | * folding rxns: |
| - | + | 32 (40 {{ul}} rxn) c5.0C (no latch, inside ligand) | |
| - | + | 32 (40 {{ul}} rxn) c5.0D (no latch, outside ligand) | |
| + | 8 (40 {{ul}} rxn) c5.0A (no latch, no ligand) | ||
* repeat 8%, 10% PEG precipitation | * repeat 8%, 10% PEG precipitation | ||
Revision as of 10:48, 16 August 2006
Repeat PEG Precipitation + Protection Assay
Overview
- concentrate on 30 mM MgCl2 folding buffer, 1x oligos trial from yesterday (yielded best PEG results)
- folding rxns:
32 (40 μL rxn) c5.0C (no latch, inside ligand)
32 (40 μL rxn) c5.0D (no latch, outside ligand)
8 (40 μL rxn) c5.0A (no latch, no ligand)
- repeat 8%, 10% PEG precipitation
