IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-17

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Revision as of 11:17, 17 August 2006

2nd PEG Precipitation

  • conduct 2nd PEG precipitation on yesterday's precipitated samples (5.0A, C, D 7%-10%)
  • protocol: mix all ingredients, add nanostructures last, total volume is 100 mL, incubate on ice for 15 min, spin for 10 min at 16k rcf, pipet off supernatant, resuspend pellet in 100 μL water
Final PEG %PEG μLNaCl μLc5.0 μLdH2O μL
735104015
840104010
94510405
105010400


lane contents loading dye
18 μL untreated 5.0.A3 μL
220 μL 5.0.A in 7% PEG and 0.5 M NaCl (supernatant)3 μL
38 μL 5.0.A in 7% PEG and 0.5 M NaCl (pellet)3 μL
420 μL 5.0.A in 8% PEG and 0.5 M NaCl (supernatant)3 μL
58 μL 5.0.A in 8% PEG and 0.5 M NaCl (pellet)3 μL
620 μL 5.0.A in 9% PEG and 0.5 M NaCl (supernatant)3 μL
78 μL 5.0.A in 9% PEG and 0.5 M NaCl (pellet)3 μL
820 μL 5.0.A in 10% PEG and 0.5 M NaCl (supernatant)3 μL
98 μL 5.0.A in 10% PEG and 0.5 M NaCl (pellet)3 μL
101 kb+ ladder3 μL
112.7 μL p73083 μL
128 μL untreated 5.0.C3 μL
1320 μL 5.0.C in 7% PEG and 0.5 M NaCl (supernatant)3 μL
148 μL 5.0.C in 7% PEG and 0.5 M NaCl (pellet)3 μL
1520 μL 5.0.C in 8% PEG and 0.5 M NaCl (supernatant)3 μL
168 μL 5.0.C in 8% PEG and 0.5 M NaCl (pellet)3 μL
1720 μL 5.0.C in 9% PEG and 0.5 M NaCl (supernatant)3 μL
188 μL 5.0.C in 9% PEG and 0.5 M NaCl (pellet)3 μL
1920 μL 5.0.C in 10% PEG and 0.5 M NaCl (supernatant)3 μL
208 μL 5.0.C in 10% PEG and 0.5 M NaCl (pellet)3 μL
218 μL untreated 5.0.D3 μL
2220 μL 5.0.D in 7% PEG and 0.5 M NaCl (supernatant)3 μL
238 μL 5.0.D in 7% PEG and 0.5 M NaCl (pellet)3 μL
2420 μL 5.0.D in 8% PEG and 0.5 M NaCl (supernatant)3 μL
258 μL 5.0.D in 8% PEG and 0.5 M NaCl (pellet)3 μL
2620 μL 5.0.D in 9% PEG and 0.5 M NaCl (supernatant)3 μL
278 μL 5.0.D in 9% PEG and 0.5 M NaCl (pellet)3 μL
2820 μL 5.0.D in 10% PEG and 0.5 M NaCl (supernatant)3 μL
298 μL 5.0.D in 10% PEG and 0.5 M NaCl (pellet)3 μL
301 kb+ ladder3 μL
312.7 μL p73083 μL
32-40(empty)3 μL
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