IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-18: Difference between revisions
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* Mixed c5.0.1 and other pre-working stocks and working stocks listed above. | * Mixed c5.0.1 and other pre-working stocks and working stocks listed above. | ||
* Folded 4 rxns each | * Folded 4 rxns each, at intermediate {Mgcl2}} of 20mM final concentration, of: | ||
** E(b) | ** E(b) | ||
** F(b) | ** F(b) |
Revision as of 13:29, 19 August 2006
Streptavidin Magnetic Beads, Take 3-4
- Trypsinized the beads, in 50uL solution that were retained from yesterday, for four hours @ 37[[:Category:{{{1}}}|{{{1}}}]].
- Ran gel of them, ladder, and p7308
- Noticed when took the gel out for imaging that the buffer was below the gel slightly - didn't cover top
- Imaging: NO BANDS AT ALL, not for the ladder or even p7308.
- Suspect that the gel didn't run due to too-low-buffer.
- On the off-chance that the trypsin digest didn't work, used 2uL (of unknown activity concentration, see above) of proteinase K to try again to digest the streptavidin.
- Incubated overnight at 37[[:Category:{{{1}}}|{{{1}}}]].
- Mixed c5.0.1 and other pre-working stocks and working stocks listed above.
- Folded 4 rxns each, at intermediate {Mgcl2}} of 20mM final concentration, of:
- E(b)
- F(b)
- A lidless
Plans for 12 Hours from Now
- Run gel of proteinase-K-ed final supernatant
- Test agarose beads on 1 rxn each of E(b), F(b), and A
- Will need ~10uL of each of these, un-beaded, for gel later too.
- Wait for magnetic beads to come in tomorrow afternoon, if they do; then use them.
Folding design 6
- Mixing pre-working stocks (c6.0.1, c6.0.2, c6.0.3, c6.0.4, c6.0.5)
- To mix pre-working stocks from plates, pipet 10 μL of each of the appropriate oligos into 1.5 mL tubes.
- List of pre-working and working stock oligos: Table_of_c6.0_Working_Stocks