< IGEM:Harvard | 2006 | DNA nanostructures | Notebook
Streptavidin Magnetic Bead Depletion Assay
- Biotinylated oligos still haven't come in yet, but the necessary accompanying "split" oligos (ie. the c5.0.#rb oligos) without biotinylation did come in.
- Reconstituted them to 200uM and put in c5.0 rack.
|oligo name||nmoles in tube||EB necessary for 200uM (uL)|
- (Previous biotinylated oligo tube orders from Invitrogen have been at ~200nmole amounts, but that has been greatly in excess of what has been actually needed and used. Thus this order was made with ~50nmole amounts, which will explain the relatively smaller amount of liquid in each tube, in case anyone is concerned about this at a later date.)