IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-30

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Streptavidin Magnetic Bead Depletion Assay

  • Biotinylated oligos still haven't come in yet, but the necessary accompanying "split" oligos (ie. the c5.0.#rb oligos) without biotinylation did come in.
  • Reconstituted them to 200uM and put in c5.0 rack.
oligo name nmoles in tube EB necessary for 200uM (uL)
c5.0.81rb44.98224.9
c5.0.8.2rb53.16265.8
c5.0.8.5rb44.72223.6
c5.0.9.1rb45.62228.1
c5.0.9.3rb40.36201.8
c5.0.9.5rb36.33181.65
    • (Previous biotinylated oligo tube orders from Invitrogen have been at ~200nmole amounts, but that has been greatly in excess of what has been actually needed and used. Thus this order was made with ~50nmole amounts, which will explain the relatively smaller amount of liquid in each tube, in case anyone is concerned about this at a later date.)
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