IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-4: Difference between revisions
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* 2uL of 10x loading dye was added to each mixture subsequently | * 2uL of 10x loading dye was added to each mixture subsequently | ||
* gel was run at 4°C for 1hr 10minutes at 100V | * gel was run at 4°C for 1hr 10minutes at 100V | ||
* gel was stained in coomassie blue from 12PM F 8/4/06 to ________ | |||
{| {{table}} | {| {{table}} | ||
Revision as of 09:21, 4 August 2006
PAGE of Concentrated Eb, Fb, and Gb
- given amounts of streptavidin were added to each component on parafilm
- 2uL of 10x loading dye was added to each mixture subsequently
- gel was run at 4°C for 1hr 10minutes at 100V
- gel was stained in coomassie blue from 12PM F 8/4/06 to ________
| lane | component | concentration | amount of component in well μL | streptavidin added μL |
| 1 | streptavidin | 2uM | 3.5 | - |
| 2 | p7308 | 44uM | 7.5 | 3.5 |
| 3 | c4.0.6b (ie. biotinylated inside-oligos) | 50nM each oligo (as in all pre-working stocks) | 7 | 3.5 |
| 4 | Eb PEG-pellet | 21/8 of a rxn | 15 | 3.5 |
| 5 | Fb PEG-pellet | 21/8 of a rxn | 15 | 3.5 |
| 6 | Gb PEG-pellet | 21/8 of a rxn | 15 | 3.5 |
| 7 | streptavidin | 2uM | 7 | - |
