IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-5
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PAGE Gel Imaging of Superconcentration of Eb, Fb, and Gb
- imaged gel from yesterday
lane | component | concentration | amount of component in well μL | streptavidin added μL |
1 | streptavidin | 2uM | 3.5 | - |
2 | p7308 | 44uM | 7.5 | 3.5 |
3 | c4.0.6b (ie. biotinylated inside-oligos) | 50nM each oligo (as in all pre-working stocks) | 7 | 3.5 |
4 | Eb PEG-pellet | 21/8 of a rxn | 15 | 3.5 |
5 | Fb PEG-pellet | 21/8 of a rxn | 15 | 3.5 |
6 | Gb PEG-pellet | 21/8 of a rxn | 15 | 3.5 |
7 | streptavidin | 2uM | 7 | - |