IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-8: Difference between revisions
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*c6.0B (in aptamers) | *c6.0B (in aptamers) | ||
*c6.0C (out aptamers) | *c6.0C (out aptamers) | ||
====Protocol==== | |||
Each reaction: | |||
*9 {{ul}} p7308 scaffold (44 nM) | |||
*16 {{ul}} oligo stock (250 nM) | |||
*4 {{ul}} 10x folding buffer (500 mM HEPES pH 7.5, 500 mM NaCl, 100 mM {{mgcl2}}) | |||
*11 {{ul}} d{{h2o}} | |||
Folding conditions: | |||
*start at 80{{c}}, decrement 1{{c}} every 2 min. for 60 cycles. | |||
====Gel Analysis==== | |||
*Run 2% agarose gel, supplemented to 10 mM {{mgcl2}} | |||
*Run in 1x TBE supplemented to 10 mM {{mgcl2}} | |||
Revision as of 12:14, 8 August 2006
Folding Rxns w/ New Ligands
- c3.2F (latch 1, in aptamers)
- c3.2G (latch 1, out aptamers)
- c3.2H (latch 2, in aptamers)
- c3.2I (latch 2, out aptamers)
- c4.0F (latch 1, in atpamers)
- c4.0G (latch 1, out aptamers)
- c4.0H (latch 2, in aptamers)
- c4.0I (latch 2, out aptamers)
- c5.0C (latch 2, in aptamers)
- c5.0D (latch 2, out aptamers)
- c6.0A (no aptamers)
- c6.0B (in aptamers)
- c6.0C (out aptamers)
Protocol
Each reaction:
- 9 μL p7308 scaffold (44 nM)
- 16 μL oligo stock (250 nM)
- 4 μL 10x folding buffer (500 mM HEPES pH 7.5, 500 mM NaCl, 100 mM MgCl2)
- 11 μL dH2O
Folding conditions:
- start at 80[[:Category:{{{1}}}|{{{1}}}]], decrement 1[[:Category:{{{1}}}|{{{1}}}]] every 2 min. for 60 cycles.
Gel Analysis
- Run 2% agarose gel, supplemented to 10 mM MgCl2
- Run in 1x TBE supplemented to 10 mM MgCl2
