IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-8: Difference between revisions
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==Folding Rxns w/ New Ligands== | ==Folding Rxns w/ New Ligands | ||
====Working stocks==== | |||
[[IGEM:Harvard/2006/DNA_nanostructures/Container_Design_3#Working_stocks|Design 3]] | [[IGEM:Harvard/2006/DNA_nanostructures/Container_Design_3#Working_stocks|Design 3]] | ||
*c3.2F (latch 1, in aptamers) | *c3.2F (latch 1, in aptamers) |
Revision as of 12:23, 8 August 2006
==Folding Rxns w/ New Ligands
Working stocks
- c3.2F (latch 1, in aptamers)
- c3.2G (latch 1, out aptamers)
- c3.2H (latch 2, in aptamers)
- c3.2I (latch 2, out aptamers)
- c4.0F (latch 1, in atpamers)
- c4.0G (latch 1, out aptamers)
- c4.0H (latch 2, in aptamers)
- c4.0I (latch 2, out aptamers)
- c5.0C (latch 2, in aptamers)
- c5.0D (latch 2, out aptamers)
- c6.0A (no aptamers)
- c6.0B (in aptamers)
- c6.0C (out aptamers)
Protocol
Each reaction:
- 9 μL p7308 scaffold (44 nM)
- 16 μL oligo stock (250 nM)
- 4 μL 10x folding buffer (500 mM HEPES pH 7.5, 500 mM NaCl, 100 mM MgCl2)
- 11 μL dH2O
Folding conditions:
- start at 80[[:Category:{{{1}}}|{{{1}}}]], decrement 1[[:Category:{{{1}}}|{{{1}}}]] every 2 min. for 60 cycles.
Gel Analysis
- Run 2% agarose gel, supplemented to 10 mM MgCl2
- Run in 1x TBE supplemented to 10 mM MgCl2