IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-8

Folding Rxns w/ New Ligands

Design 3

• c3.2F (latch 1, in aptamers)
• c3.2G (latch 1, out aptamers)
• c3.2H (latch 2, in aptamers)
• c3.2I (latch 2, out aptamers)

Design 4

• c4.0F (latch 1, in atpamers)
• c4.0G (latch 1, out aptamers)
• c4.0H (latch 2, in aptamers)
• c4.0I (latch 2, out aptamers)

Design 5

• c5.0B (latch 2, in aptamers)
• c5.0C (latch 2, out aptamers)

Design 6

• c6.0A (no aptamers)
• c6.0B (in aptamers)
• c6.0C (out aptamers)

Description	Contents	Total
c4.0.4L: barrel oligos @ outside aptamers + ligand	4.0.4.1oa/ob, 4.0.4.2oa/ob, 4.0.4.3oa/ob, 4.0.4.4oa/ob	8
c4.0.6L: barrel oligos @ inside aptamers + ligand	4.0.6.1oa/ob, 4.0.6.2oa/ob, 4.0.6.3oa2/ob2, 4.0.6.4oa2/ob2	8

(mix 5 μL of each 100 μM stock ligand-oligos to make pre-working stocks)

Working Stocks	Description	Pre-Working Stocks	Water	Total
c4.0FL.core	latch design 1, inside aptamers	1 (66 μL), 2 (33 μL), 3 (33 μL), 4 (4 μL), 6L (8 μL)	48 μL	200 μL
c4.0FL.latches	latch design 1, inside aptamers	8 (4 μL), 11 (2 μL), 12 (2 μL)	-	-
c4.0GL.core	latch design 1, outside aptamers	1 (66 μL), 2 (33 μL), 3 (33 μL), 4L (8 μL), 6 (4 μL)	48 μL	200 μL
c4.0GL.latches	latch design 1, outside aptamers	8 (4 μL), 11 (2 μL), 12 (2 μL)	-	-
c4.0HL.core	latch design 2, inside aptamers	1 (66 μL), 2 (33 μL), 3 (33 μL), 4 (4 μL), 6L (8 μL)	40 μL	200 μL
c4.0HL.latches	latch design 2, inside aptamers	9 (4 μL), 13 (2 μL), 14 (2 μL), 23 (4 μL), 24 (2 μL), 25 (2 μL)	-	-
c4.0IL.core	latch design 2, outside aptamers	1 (66 μL), 2 (33 μL), 3 (33 μL), 4L (8 μL), 6 (4 μL)	40 μL	200 μL
c4.0IL.latches	latch design 2, outside aptamers	9 (4 μL), 13 (2 μL), 14 (2 μL), 23 (4 μL), 24 (2 μL), 25 (2 μL)	-	-