IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-8: Difference between revisions

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*c6.0B (in aptamers)
*c6.0B (in aptamers)
*c6.0C (out aptamers)
*c6.0C (out aptamers)
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Description'''
| align="center" style="background:#f0f0f0;"|'''Contents'''
| align="center" style="background:#f0f0f0;"|'''Total'''
|-
|c4.0.4L: barrel oligos @ outside aptamers + ligand||4.0.4.1oa/ob, 4.0.4.2oa/ob, 4.0.4.3oa/ob, 4.0.4.4oa/ob||8
|-
|c4.0.6L: barrel oligos @ inside aptamers + ligand||4.0.6.1oa/ob, 4.0.6.2oa/ob, 4.0.6.3oa2/ob2, 4.0.6.4oa2/ob2||8
|-
|}
(mix 5 {{ul}} of each 100 {{uM}} stock ligand-oligos to make pre-working stocks)
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Working Stocks'''
| align="center" style="background:#f0f0f0;"|'''Description'''
| align="center" style="background:#f0f0f0;"|'''Pre-Working Stocks'''
| align="center" style="background:#f0f0f0;"|'''Water'''
| align="center" style="background:#f0f0f0;"|'''Total'''
|-
|c4.0FL.core||latch design 1, inside aptamers||1 (66 {{ul}}), 2 (33 {{ul}}), 3 (33 {{ul}}), 4 (4 {{ul}}), 6L (8 {{ul}})||48 {{ul}}||200 {{ul}}
|-
|c4.0FL.latches||latch design 1, inside aptamers||8 (4 {{ul}}), 11 (2 {{ul}}), 12 (2 {{ul}})||-||-
|-
|c4.0GL.core||latch design 1, outside aptamers||1 (66 {{ul}}), 2 (33 {{ul}}), 3 (33 {{ul}}), 4L (8 {{ul}}), 6 (4 {{ul}})||48 {{ul}}||200 {{ul}}
|-
|c4.0GL.latches||latch design 1, outside aptamers||8 (4 {{ul}}), 11 (2 {{ul}}), 12 (2 {{ul}})||-||-
|-
|c4.0HL.core||latch design 2, inside aptamers||1 (66 {{ul}}), 2 (33 {{ul}}), 3 (33 {{ul}}), 4 (4 {{ul}}), 6L (8 {{ul}})||40 {{ul}}||200 {{ul}}
|-
|c4.0HL.latches||latch design 2, inside aptamers||9 (4 {{ul}}), 13 (2 {{ul}}), 14 (2 {{ul}}), 23 (4 {{ul}}), 24 (2 {{ul}}), 25 (2 {{ul}})||-||-
|-
|c4.0IL.core||latch design 2, outside aptamers||1 (66 {{ul}}), 2 (33 {{ul}}), 3 (33 {{ul}}), 4L (8 {{ul}}), 6 (4 {{ul}})||40 {{ul}}||200 {{ul}}
|-
|c4.0IL.latches||latch design 2, outside aptamers||9 (4 {{ul}}), 13 (2 {{ul}}), 14 (2 {{ul}}), 23 (4 {{ul}}), 24 (2 {{ul}}), 25 (2 {{ul}})||-||-
|}

Revision as of 08:34, 8 August 2006

Folding Rxns w/ New Ligands

Design 3

  • c3.2F (latch 1, in aptamers)
  • c3.2G (latch 1, out aptamers)
  • c3.2H (latch 2, in aptamers)
  • c3.2I (latch 2, out aptamers)

Design 4

  • c4.0F (latch 1, in atpamers)
  • c4.0G (latch 1, out aptamers)
  • c4.0H (latch 2, in aptamers)
  • c4.0I (latch 2, out aptamers)

Design 5

  • c5.0B (latch 2, in aptamers)
  • c5.0C (latch 2, out aptamers)

Design 6

  • c6.0A (no aptamers)
  • c6.0B (in aptamers)
  • c6.0C (out aptamers)
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