IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-8
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Folding Rxns w/ New Ligands
- c3.2F (latch 1, in aptamers)
- c3.2G (latch 1, out aptamers)
- c3.2H (latch 2, in aptamers)
- c3.2I (latch 2, out aptamers)
Final concentration of each oligo in working stocks is 250 nM.
| working stock | description | pre-working stocks (according to chart) | water | total |
| c3.2.Fo.core | +latch1 +aptamers_in |
1 (94 μL), 2 (28 μL), 3 (30 μL), 5 (3 μL), 6o (5 μL), 10 (2 μL), 11 (2 μL), 13 (0 μL) | 36 μL | 200 μL |
| c3.2.Fo.latches | +latch1 +aptamers_in |
14 (2 μL) | 10.5 μL | 12.5 μL of 4 μM |
| c3.2.Go.core | +latch1 +aptamers_out |
1 (94 μL), 2 (28 μL), 3 (30 μL), 4 (3 μL), 7o (4 μL), 10 (2 μL), 11 (2 μL), 13 (0 μL) | 37 μL | 200 μL |
| c3.2.Go.latches | +latch1 +aptamers_out |
14 (2 μL) | 10.5 μL | 12.5 μL of 4 μM |
| c3.2.Ho.core | +latch2 +aptamers_in |
1 (94 μL), 2 (28 μL), 3 (30 μL), 5 (3 μL), 6o (5 μL), 10 (2 μL), 12 (2 μL), 13 (0 μL), 15 (2 μL) | 34 μL | 200 μL |
| c3.2.Ho.latches | +latch2 +aptamers_in |
16 (4 μL) | 21 μL | 25 μL of 2 μM |
| c3.2.Io.core | +latch2 +aptamers_out |
1 (94 μL), 2 (28 μL), 3 (30 μL), 4 (3 μL), 7o (4 μL), 10 (2 μL), 12 (2 μL), 13 (0 μL), 15 (2 μL) | 35 μL | 200 μL |
| c3.2.Io.latches | +latch2 +aptamers_out |
16 (4 μL) | 21 μL | 25 μL of 2 μM |
- c4.0F (latch 1, in atpamers)
- c4.0G (latch 1, out aptamers)
- c4.0H (latch 2, in aptamers)
- c4.0I (latch 2, out aptamers)
- c5.0C (latch 2, in aptamers)
- c5.0D (latch 2, out aptamers)
- c6.0A (no aptamers)
- c6.0B (in aptamers)
- c6.0C (out aptamers)
