IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-8-9: Difference between revisions
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==Folding reaction== | |||
Fold 200 {{ul}} each of 3.2.Eo, .Fo, .Go, .Ho | |||
Each reaction: | |||
*45 {{ul}} p7308 scaffold (44 nM) | |||
*80 {{ul}} oligo stock (250 nM) | |||
*20 {{ul}} 10x folding buffer (500 mM HEPES pH 7.5, 500 mM NaCl, 100 mM {{mgcl2}}) | |||
*55 {{ul}} d{{h2o}} | |||
Folding conditions: | |||
*high-volume protocol: start at 80{{c}}, decrement 0.5{{c}} every 1 min. for 120 cycles. | |||
==More Microcon trials== | ==More Microcon trials== | ||
==Microcon purification of yesterday's folding reactions== | ==Microcon purification of yesterday's folding reactions== | ||
Revision as of 10:49, 9 August 2006
Folding reaction
Fold 200 μL each of 3.2.Eo, .Fo, .Go, .Ho
Each reaction:
- 45 μL p7308 scaffold (44 nM)
- 80 μL oligo stock (250 nM)
- 20 μL 10x folding buffer (500 mM HEPES pH 7.5, 500 mM NaCl, 100 mM MgCl2)
- 55 μL dH2O
Folding conditions:
- high-volume protocol: start at 80[[:Category:{{{1}}}|{{{1}}}]], decrement 0.5[[:Category:{{{1}}}|{{{1}}}]] every 1 min. for 120 cycles.
