IGEM:Harvard/2006/DNA nanostructures/Notebook/2006-9-12: Difference between revisions
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* We only need 10 new oligos, without biotinylation obviously, so they're not too expensive - better to have them on hand when we need them than have to wait for them later. | * We only need 10 new oligos, without biotinylation obviously, so they're not too expensive - better to have them on hand when we need them than have to wait for them later. | ||
* Used the convention from c4.0 of: '''original (split) sequence (taken from the sequences of the biotinylated revised oligos c5.0.8r and c5.0.9r) | * Used the convention from c4.0 of: '''original (split) sequence''' (taken from the sequences of the biotinylated revised oligos c5.0.8r and c5.0.9r), '''3Ts''', followed by the thrombin-aptamer sequence '''ggttggtgtggttgg'''. | ||
* See [[IGEM:Harvard/2006/Container_Design_5/Oligos#Aptamer_Oligos_.289.2F12.29|Notebook on 9.12]] for the sequences ordered, and what oligos from older orders will be needed to add to them. | * See [[IGEM:Harvard/2006/Container_Design_5/Oligos#Aptamer_Oligos_.289.2F12.29|Notebook on 9.12]] for the sequences ordered, and what oligos from older orders will be needed to add to them. | ||
* Order was sent to Alain 4:55PM 9.12.06. | |||
Latest revision as of 13:55, 12 September 2006
Designed and Ordered Aptamer Oligos for c5.0
- Since the streptavidin-biotinylation protection assay showed success, and since our original plan was to work with a more in vivo useful biological pairing (ie. thrombin to thrombin-aptamer), I thought it would be a good idea to order the appropriate thrombin-aptamer-containing oligos for a protection assay.
- We only need 10 new oligos, without biotinylation obviously, so they're not too expensive - better to have them on hand when we need them than have to wait for them later.
- Used the convention from c4.0 of: original (split) sequence (taken from the sequences of the biotinylated revised oligos c5.0.8r and c5.0.9r), 3Ts, followed by the thrombin-aptamer sequence ggttggtgtggttgg.
- See Notebook on 9.12 for the sequences ordered, and what oligos from older orders will be needed to add to them.
- Order was sent to Alain 4:55PM 9.12.06.
